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M9460274.TXT
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1994-06-12
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Document 0274
DOCN M9460274
TI Evidence for simian immunodeficiency virus-specific IgM and IgG response
in peripheral blood mononuclear cells of serum enzyme-linked
immunosorbent assay-negative nonhuman primates.
DT 9408
AU Jehuda-Cohen T; Powell JD; Villinger F; Mayne AE; Sell KW; Ansari AA;
Department of Pathology and Laboratory Medicine, Winship Cancer; Center,
Emory University School of Medicine, Atlanta, Georgia; 30322.
SO J Acquir Immune Defic Syndr. 1994 Jun;7(6):539-50. Unique Identifier :
AIDSLINE MED/94231458
AB In vitro polyclonal activation of peripheral blood mononuclear cells
(PBMCs) from 70% of the simian immunodeficiency virus (SIV) serum
enzyme-linked, immunosorbent assay (ELISA)-negative sooty mangabeys
leads to synthesis and release of low but significant and reproducible
levels of SIV-reactive antibodies, as determined by ELISA and Western
blot analysis. The predominant isotype of SIV-reactive antibodies in the
pokeweed mitogen (PWM) supernatant fluids from serum ELISA-negative
mangabeys is IgM, whereas the predominant isotype of SIV-reactive
antibodies in seropositive mangabeys is IgG. Depletion of CD8+ cells led
to a marked increase in the levels of SIV-reactive antibodies detected
in supernatant fluids from PWM-induced cultures from the serum
ELISA-negative mangabeys. No evidence for such SIV-reactive antibodies
has been found, to date, in similar unfractionated or CD8+
T-cell-depleted PWM-induced PBMC cultures from uninfected macaques.
Supernatant fluids from PWM cultures of PBMCs from a select group of
serum ELISA-negative mangabeys, when concentrated five times, were shown
to give a Western blot profile against SIV, similar to the profile seen
with plasma from seropositive infected macaques and mangabeys. Evidence
is presented to show that these serum ELISA-negative mangabeys are most
likely latently infected with SIV. This evidence, which was obtained in
samples from such ELISA-negative mangabeys, includes the detection of
reverse transcriptase activity and the presence of SIV p27 in
supernatant fluids of phytohemagglutinin-stimulated PBMCs in vitro. In
addition, the data show the presence of CD8+ T cells that regulate
SIV-specific Ig synthesis and show the detection of gag sequences by the
polymerase chain reaction. Thus, the PWM assay described herein may
provide a valuable additional tool for detection of lentivirus infection
before or in the absence of seroconversion.
DE Animal Antibodies, Viral/BIOSYNTHESIS Antibody Specificity Blotting,
Western Cells, Cultured Cercocebus atys Enzyme-Linked Immunosorbent
Assay IgG/*BIOSYNTHESIS IgM/*BIOSYNTHESIS Leukocytes,
Mononuclear/IMMUNOLOGY/*MICROBIOLOGY Macaca mulatta Simian Acquired
Immunodeficiency Syndrome/DIAGNOSIS/*IMMUNOLOGY Support, Non-U.S. Gov't
Support, U.S. Gov't, P.H.S. SIV/*IMMUNOLOGY/PHYSIOLOGY T-Lymphocytes,
Suppressor-Effector/IMMUNOLOGY Virus Latency JOURNAL ARTICLE
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).